Coronary Circulation Enhances the Aerobic Performance of Wild Pacific Salmon
Data files
Jun 06, 2024 version files 25.63 KB
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README.md
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VanWert_etal_2024_coho_bloodchem.csv
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VanWert_etal_2024_coho_metabolism.csv
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VanWert_etal_2024_coho_pvo2.csv
5.86 KB
Jun 07, 2024 version files 25.61 KB
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README.md
5.13 KB
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VanWert_etal_2024_coho_bloodchem.csv
10.23 KB
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VanWert_etal_2024_coho_metabolism.csv
4.39 KB
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VanWert_etal_2024_coho_pvo2.csv
5.86 KB
Abstract
Female Pacific salmon often experience higher mortality than males during their once-in-a-lifetime up-river spawning migration, particularly when exposed to secondary stressors (e.g. high temperatures). However, the underlying mechanisms remain unknown. One hypothesis is that female Pacific salmon hearts are more oxygen-limited than males and are less able to supply oxygen to the body’s tissues during this demanding migration. Notably, female hearts have higher coronary blood flow, which could indicate a greater reliance on this oxygen source. Oxygen limitations can develop from naturally occurring coronary blockages (i.e., coronary arteriosclerosis) found in mature salmon hearts. If female hearts rely more heavily on coronary blood flow but experience similar arteriosclerosis levels as males, they will have disproportionately impaired aerobic performance. To test this hypothesis, we measured resting (RMR) and maximum metabolic rate (MMR), aerobic scope (AS) and acute upper thermal tolerance in coho salmon (Oncorhynchus kisutch) with an intact or artificially blocked coronary oxygen supply. We also assessed venous blood oxygen and chemistry (cortisol, ions, and metabolite concentrations) at different time intervals during recovery from exhaustive exercise. We found that coronary blockage impaired MMR, AS, and the partial pressure of oxygen in venous blood (PvO2) during exercise recovery but did not differ between sexes. Coronary ligation lowered acute upper thermal tolerance by 1.1°C. Though we did not find evidence of enhanced female reliance on coronary supply, our findings highlight the importance of coronary blood supply for mature wild salmon, where migration success may be linked to cardiac performance, particularly during warm water conditions.
README: Van Wert et al 2024
Coronary circulation enhances the aerobic performance of wild Pacific salmon
Description of the Data and file structure
Author Information
A. Principal Investigator Contact Information
Name: Dr. Erika Eliason or Jacey Van Wert
Institution: University of California, Santa Barbara
Email: eliason@ucsb.edu, jcvanwert@gmail.com
B. Corresponding Author Contact Information
Name: Jacey Van Wert
Institution: University of California, Santa Barbara
Email: jcvanwert@gmail.com
Date range of data collection (single date, range, approximate date): Oct - Nov 2019
Geographic location of data collection: Cultus Lake Laboratory, Chilliwack, BC, Canada
Information about funding sources that supported the collection of the data:
This work was supported by the US National Science Foundation [Graduate Research Fellowship support to JCV]; the Swedish Research Council [2018-00516 to AE]; the University of California, Santa Barbara [Faculty Research Grant to EJE]; Natural Sciences and Engineering Research Council of Canada [Collaborative Research Development Grant to SGH; Discovery Grant to SGH and SJC]; Department of Fisheries and Oceans Canada [Environmental Watch Program to DAP].
File List:
- VanWert_etal_2024_coho_metabolism.csv - contains all fish id's with morphology and metabolism metrics
- VanWert_etal_2024_coho_pvo2.csv - contains all fish id's with blood partial pressure of venous oxygen at various sampling timepoints
- VanWert_etal_2024_coho_bloodchem.csv - contains all fish id's with blood chemistry at various sampling timepoints
METHODOLOGICAL INFORMATION
- Description of methods used for collection/generation/processing of data: Please refer to the main manuscript for details regarding the methods used to collect and analyze this data.
- People involved with sample collection, processing, analysis and/or submission:Jacey C. Van Wert, Andreas Ekström, Matthew J.H. Gilbert, Brian J. Hendriks, Steven J. Cooke, David A. Patterson, Scott G. Hinch and Erika J. Eliason
DATA-SPECIFIC INFORMATION FOR: VanWert_etal_2024_coho_metabolism.csv
- Number of variables: 21
- Number of cases/rows: 42
- Variable List:
id = unique fish ID
weight_kg = body mass (kg)
respo_vol_l = respirometer volume (l)
treatment = sham operated (sham) or coronary ligated (ligated)
sex = male (m) or female (f)
premature_euth_pre_ctmax = whether fish was euthanized before reaching the beginning of the ctmax test due to indications of bleeding from the cannula with y = yes, n = no
adipose = whether adipose fin is present or not, clipped indicates hatchery origin with y = yes, n = no
heart_g = wet ventricle mass (g)
liver_g = wet liver mass (g)
gonad_g = wet gonad mass (g)
spleen_g = wet spleen mass (g)
fork_cm = fork length (cm)
percent_compact = percent compact myocardium that comprises the ventricle(%)
ctmax = critical thermal maximum temperature (CTmax) (ramping rate specified in publication) in Celsius
rbc_ctmax = red blood cell proportion measured at CTmax
mmr = maximum metabolic rate (mg O2 / kg/min)
rmr = resting metabolic rate (mg O2 / kg/min)
aas = absolute aerobic scope measured by mmr-rmr (mg O2 / kg/min)
fas = factorial aerobic scope measured by mmr/rmr (unitless)
epoc = excess post oxygen consumption (mg/kg), calculated as the area under the curve until reaching rmr, minus the area under rmr
epoc_dur = duration of epoc (min)
DATA-SPECIFIC INFORMATION FOR: VanWert_etal_2024_coho_pvo2.csv
- Number of variables: 5
- Number of cases/rows: 206
- Variable List:
fish_id = unique fish ID
treatment = sham operated (sham) or coronary ligated (ligated)
sex = male (m) or female (f)
timepoint = time at which partial pressure of venous oxygen (pvo2) was sampled; with time0 immediately after the exhaustive chase, time15 15 min after chase, time60 60 min after chase, rest at 18 h after chase with minimal disturbances, and ctmax immediately after ctmax trial
pvo2_torr = partial pressure of oxygen (torr) associated with the sampling timepoint and fish_id
DATA-SPECIFIC INFORMATION FOR: VanWert_etal_2024_coho_bloodchem.csv
- Number of variables: 10
- Number of cases/rows: 206
- Variable List:
fish_id = unique fish ID
treatment = sham operated (sham) or coronary ligated (ligated)
sex = male (m) or female (f)
timepoint = time at which blood was sampled; with 0 immediately after the exhaustive chase, 15 min after chase, 60 min after chase, rest at 18 h after chase with minimal disturbances, and ctmax immediately after ctmax trial
glucose_mmol_l = plasma glucose (mmol/l)
lactate_mmol_l = plasma lactate (mmol/l)
na_mmol_l = plasma sodium (mmol/l)
k_mmol_l = plasma potassium (mmol/l)
cortisol_ng_ml = plasma cortisol (ng/ml)
ht = mean of plasma hematocrit (%)
Sharing/access Information
- Licenses/restrictions placed on the data: N/A
- Links to publications that cite or use the data: N/A
- Links to other publicly accessible locations of the data: N/A
- Links/relationships to ancillary data sets: N/A
- Was data derived from another source? No
Methods
Adult coho salmon (Oncorhynchus kisutch) were collected at Chilliwack Hatchery, British Columbia, Canada and fish were held at Cultus Lake Laboratory for experiments. See publication for methodology details.