Images of loop-mediated isothermal amplification (LAMP) for SARS-CoV-2 testing and optimized 'Cap-iLAMP'

Riesenberg, Stephan, Max Planck Institute for Evolutionary Anthropology, https://orcid.org/0000-0001-8171-1717

Bokelmann, Lukas, Max Planck Institute for Evolutionary Anthropology

Maricic, Tomislav, Max Planck Institute for Evolutionary Anthropology

Pääbo, Svante, Max Planck Institute for Evolutionary Anthropology

Meyer, Matthias, Max Planck Institute for Evolutionary Anthropology

Nickel, Olaf, Klinikum St. Georg

Borte, Stephan, Klinikum St. Georg

stephan_riesenberg@eva.mpg.de

Published Jan 28, 2021 on Dryad. https://doi.org/10.5061/dryad.2rbnzs7mk

Cite this dataset

Riesenberg, Stephan et al. (2021). Images of loop-mediated isothermal amplification (LAMP) for SARS-CoV-2 testing and optimized 'Cap-iLAMP' [Dataset]. Dryad. https://doi.org/10.5061/dryad.2rbnzs7mk

Abstract

Here we present images of loop-mediated isothermal amplification (LAMP) and optimized Cap-iLAMP (capture and improved ‎loop-mediated isothermal amplification). Cap-iLAMP combines a hybridization capture-based RNA extraction of gargle lavage samples with an improved colorimetric RT-LAMP assay and smartphone-based color scoring. Cap-iLAMP is compatible with point-of-care testing and enables the detection of SARS-CoV-2 positive samples in less than one hour. The sensitivity is 97% and the specificity is 99%.

Funding

Max Planck Society, NOMIS foundation, Immuno Deficiency Center Leipzig