Data for: Natural genetic variation in a dopamine receptor is associated with variation in female fertility in Drosophila melanogaster
Cite this dataset
Lyman, Richard; Huang, Wen (2023). Data for: Natural genetic variation in a dopamine receptor is associated with variation in female fertility in Drosophila melanogaster [Dataset]. Dryad. https://doi.org/10.5061/dryad.2z34tmpr6
Abstract
Fertility is a major component of fitness but its genetic architecture remains poorly understood. Using a full diallel cross of 50 Drosophila melanogaster Genetic Reference Panel inbred lines with whole genome sequences, we found substantial genetic variation in fertility largely attributable to females. We mapped genes associated with variation in female fertility by genome-wide association analysis of common variants in the fly genome. Validation of candidate genes by RNAi knockdown confirmed the role of the dopamine 2-like receptor (Dop2R) in promoting egg laying. We replicated the Dop2R effect in an independently collected productivity dataset and showed that the effect of the Dop2R variant was mediated in part by regulatory gene expression variation. This study demonstrates the strong potential of genome-wide association analysis in this diverse panel of inbred strains and subsequent functional analyses for understanding the genetic architecture of fitness traits.
Usage notes
Please refer to https://github.com/qgg-lab/dgrp-diallel for computer codes.
Funding
National Institute of General Medical Sciences, Award: R01GM45146