Bacterial leaf blight incited by Pseudomonas cannabina pv. alisalensis (Pca) is a devastating disease with incidence reports worldwide and a wide host range capable of infecting all commercially valuable Brassica crops. With no chemical control options available, the most effective form of disease control is host plant resistance, but thus far resistant germplasm has only been identified in Brassica juncea L. (mustard greens). We report the first screening of Brassica oleracea L. var. viridis germplasm, including leafy green collard and collard-like accessions, for resistance to bacterial leaf blight by artificial inoculation of Pca in greenhouse trials. All commercial cultivars tested displayed an intermediate disease response resulting in leaf lesion development that renders the product unmarketable. Two sources of significant resistance were identified in the U.S. Department of Agriculture (USDA) viridis collection, which provides a valuable source of resistance alleles for collard cultivar development and introgression into other B. oleracea crops.
accession_info
Accession information for the PIs tested, including taxonomic classification, GRIN identifiers, common names, and geographic collection locations.
stats_screenA
The USDA collard collection was screened for resistance to Pseudomonas cannabina pv alisalensis (bacterial leaf blight) through a series of five tests (A-E) using an augmented randomized complete block design. The stats files (screenA-E) report the results of an analysis of variance, Fisher's LSD, and line means for each test.
stats_screenB
The USDA collard collection was screened for resistance to Pseudomonas cannabina pv alisalensis (bacterial leaf blight) through a series of five tests (A-E) using an augmented randomized complete block design. The stats files (screenA-E) report the results of an analysis of variance, Fisher's LSD, and line means for each test.
stats_screenC
The USDA collard collection was screened for resistance to Pseudomonas cannabina pv alisalensis (bacterial leaf blight) through a series of five tests (A-E) using an augmented randomized complete block design. The stats files (screenA-E) report the results of an analysis of variance, Fisher's LSD, and line means for each test.
stats_screenD
The USDA collard collection was screened for resistance to Pseudomonas cannabina pv alisalensis (bacterial leaf blight) through a series of five tests (A-E) using an augmented randomized complete block design. The stats files (screenA-E) report the results of an analysis of variance, Fisher's LSD, and line means for each test.
stats_screenE
The USDA collard collection was screened for resistance to Pseudomonas cannabina pv alisalensis (bacterial leaf blight) through a series of five tests (A-E) using an augmented randomized complete block design. The stats files (screenA-E) report the results of an analysis of variance, Fisher's LSD, and line means for each test.
stats_retestF
Putatively resistant accessions from the initial screening were inoculated with Pseudomonas cannabina pv alisalensis for two additional greenhouse tests (retests F and H). The stats files (retestF, H, FH) report the results of an analysis of variance, Fisher's LSD, and line means for the retests separately and combined.
stats_retestH
Putatively resistant accessions from the initial screening were inoculated with Pseudomonas cannabina pv alisalensis for two additional greenhouse tests (retests F and H). The stats files (retestF, H, FH) report the results of an analysis of variance, Fisher's LSD, and line means for the retests separately and combined.
stats_retestsFH
Putatively resistant accessions from the initial screening were inoculated with Pseudomonas cannabina pv alisalensis for two additional greenhouse tests (retests F and H). The stats files (retestF, H, FH) report the results of an analysis of variance, Fisher's LSD, and line means for the retests separately and combined.