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Roles of the ClC chloride channel CLH-1 in food-associated salt chemotaxis behavior of C. elegans

Citation

Park, Chanhyun et al. (2021), Roles of the ClC chloride channel CLH-1 in food-associated salt chemotaxis behavior of C. elegans, Dryad, Dataset, https://doi.org/10.5061/dryad.4tmpg4f8c

Abstract

The ability of animals to process dynamic sensory information facilitates foraging in an ever changing environment. However, molecular and neural mechanisms underlying such ability remain elusive. The ClC anion channels/transporters play a pivotal role in cellular ion homeostasis across all phyla. Here we find a ClC chloride channel is involved in salt concentration chemotaxis of C. elegans. Genetic screening identified two altered-function mutations of clh-1 that disrupt experience-dependent salt chemotaxis. Using genetically encoded fluorescent sensors, we demonstrate that CLH-1 contributes to regulation of intracellular anion and calcium dynamics of salt-sensing neuron, ASER. The mutant CLH-1 reduced responsiveness of ASER to salt stimuli in terms of both temporal resolution and intensity, which disrupted navigation strategies for approaching preferred salt concentrations. Furthermore, other ClC genes appeared to act redundantly in salt chemotaxis. These findings provide insights into the regulatory mechanism of neuronal responsivity by ClCs that contribute to modulation of navigation behavior.

Methods

Each dataset are collected by appropriate methods. For detailed methods related to these data, please refer to "Materials and methods" of the article "Roles of the ClC chloride channel CLH-1 in food-associated salt chemotaxis behavior of C. elegans".

Usage Notes

For statistic analysis data, please refer to "Supplementary File 5 statistics" of the supplementary file of the article.

Funding

Japan Society for the Promotion of Science, Award: Grant-in-Aid for Scientific Research (S) JP17H06113

Japan Science and Technology Agency, Award: CREST JP17H06113

Japan Society for the Promotion of Science, Award: Grants-in-Aid for Innovative Area "Artificial Intelligence and Brain Science" 19H04980

University of Tokyo, Award: Center for Integrative Science of Human Behavior (CiSHuB)

Japan Society for the Promotion of Science, Award: Grant-in-Aid for Scientific Research

Japan Society for the Promotion of Science, Award: Grants-in-Aid for Innovative Area 18H04881

Japan Society for the Promotion of Science, Award: Grant-in-Aid for challenging Exploratory Research 18K19323

Japan Society for the Promotion of Science, Award: Grant-in-Aid for Scientific Research 19K06952

The Salt Science Foundation, Award: 1728

Japan Society for the Promotion of Science, Award: 19K1628

The Salt Science Foundation, Award: 2043