Data from: Harvest-associated size reductions and genomic changes within two generations in wild walleye populations
Bowles, Ella et al. (2020), Data from: Harvest-associated size reductions and genomic changes within two generations in wild walleye populations, Dryad, Dataset, https://doi.org/10.5061/dryad.5tb2rbp1z
Walleye were captured via angling using the same lures and a combination of boats and shore fishing, from the same locations within rivers, for both historical and contemporary sampling (Table 1). From each walleye, we collected total and fork length (TL ± 1 mm), wet mass (± 50 g), sex (M, F, U (unknown, either spawned out or premature)) and a tissue sample for genetics; otoliths were collected from a random subsample. Opercular bones but not otoliths were collected for aging for historic samples (Table 1), and this was done before this study, for Dupont et al. (2007); 2015 and 2017 otoliths were aged at the Wisconsin Cooperative Fishery Unit, US Geological Service, University of Wisconsin, Stevens Point, USA. Otoliths were aged by two experienced readers; if they disagreed on an age they examined the structure together to agree upon one for that structure. No walleye were aged using both opercular bones and otoliths.
DNA was extracted using a modified Qiagen blood and tissue kit protocol (Qiagen Inc., Valencia, CA) (see Table 1 for sample sizes) and was sequenced using individual-based genotyping-by-sequencing (GBS). Libraries for Ion Proton GBS were prepared using the procedure described by Masher et al. (2013) at IBIS, Université Laval, Québec, Canada, with modifications described in Abed et al. (2018). Single-nucleotide polymorphisms (SNPs) were determined from raw sequence reads using the stacks pipeline v1.45 (Catchen et al. 2013), and de novo sequence alignment.
For genomic data:
r12d files were made with a population map specifying each population and year separately, and includes outlier loci.
r12g files were made with a population map specifying Icon-Perch as a single population, and exclude outlier loci.
For body size data:
The all data file was used for body size modeling, while only the age data was used for the bayesian size-at-age models.