Co-expression of calcium and hERG potassium channels reduces the incidence of proarrhythmic events
Data files
Feb 22, 2021 version files 507.77 MB
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MEA_guide.txt
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plate1_baseline.mat
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plate1_cis.mat
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plate1_nif.mat
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plate2_baseline.mat
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plate2_cis.mat
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plate2_nif.mat
Abstract
Methods
Extracellular field potentials were recorded from hiPSC-CM monolayers using a Maestro-APEX multi-electrode array (MEA) system (Axion Biosystems, Atlanta, GA, USA). Briefly, cell monolayers were maintained for 20-30 days in STEMDiff cardiomyocte maintenance media (STEM CELL Technologies), on EStim classic 48 well MEA plates (Axion Biosystems, Atlanta, USA), prior to recording. Monolayers, maintained at 37°C, were paced at 1 Hz and signals digitized at 12.5 kHz using AxIS v2.5.1.10 software (Axion Biosystems, Atlanta, GA, USA). Field potential durations were measured from the peak depolarization spike to the peak of the repolarization wave. After 30 minutes of pacing equilibration, Electrograms were recorded for 5 minutes in control conditions, 5 minutes in the presence of cisapride (200nM), and for 5 minutes in the presence of cisparide + nifedipine (50 nM) and measurements averaged over the last 10 beats of each recording period. This allowed for the possibility that if there was any beat to beat variation this could be averaged out over the ten beats. Twelve wells were plated for each line and all wells from which signals were recorded (n=7-12) were included in the analysis.
Usage notes
MEA guide.txt (description of contents of raw data files and how to extract the data)
The data set contains 6 raw dat files (matlab files):
Plate1_baseline.mat
Plate1_cis.mat
Plate1_nif.mat
Plate2_baseline.mat
Plate2_cis.mat
Plate2_nif.mat