Spatial fingerprinting: horizontal fusion of multi-dimensional bio-tracers as solution to global food provenance problems
Data files
Apr 26, 2021 version files 27.04 KB
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biotracers.csv
Abstract
Methods
Muscle tissue trimmings were donated by Albion Farms & Fisheries Ltd. (now Intercity Packers Ltd.), Richmond, BC, Canada. All samples were derived from fillet trimmings to simulate a likely Quality Assurance/Quality Control scenario. Each muscle trimming was processed to obtain 2 muscle tissue samples for analyzing 17 bio-tracers of two classes: 3 stables isotopes (δ 15 N, δ 13C and δ 34S) and 14 fatty acids (FA). One muscle sample from each fish was delivered frozen to the Lipid Analytical Services at the University of Guelph for fatty acid analysis using a combination of Bligh & Dwyer and Morrison & Smith methods.46,47 Individual FA weights (μg/g) were converted to a % FA composition. The second muscle samples were dried at 70°C for 2 days and ground into a fine powder in preparation for stable isotope analysis. Tissue samples were sent to the University of Windsor GLIER Chemical Tracers Lab for isotopic analysis of δ 15N, δ13 C and δ 34S (Windsor, ON, Canada).
Usage notes
Columns are as follows.
- id: sample identifier
- isotopic ratios
- cal_d13C: δ13C (Carbon 13)
- cal_d15N: δ15N (Nitrogen 15)
- cal_d34S: δ34S (Sulfur 34)
- Composition of fatty acid (%, note that only fatty acids with >1% presence were retained).
- FA_C16_0: Palmitic acid (C16:0, PubChem CID: 985)
- FA_C16_1: Palmitoleic acid (C16:1, PubChem CID: 445638)
- FA_C18_0: Stearic acid (C18:0, PubChem CID: 5281)
- FA_C18_1: Oleic acid (C18:1, PubChem CID: 445639)
- FA_C18_2n6: Linoleic acid (C18:2, PubChem CID: 5280450)
- FA_C18_3n3: α-linolenic acid (C18:3, PubChem CID: 5280934)
- FA_C18_4n3: Stearidonic acid (C18:4, PubChem CID: 5312508)
- FA_C20_1: Gondoic acid (C20:1, PubChem CID: 5282768)
- FA_C20_4n3: Eicosatetraenoic acid (C20:4, PubChem CID: 21863049)
- FA_C20_5n3: Eicosapentaenoic acid (C20:5, PubChem CID: 446284)
- FA_C22_1: Erucic acid (C22:1, PubChem CID: 5281116)
- FA_C22_5n3: Docosapentaenoic acid (C22:5, PubChem CID: 5497182)
- FA_C22_6n3: Docosahexaenoic acid (C22:6, PubChem CID: 445580)
- FA_C24_1: Nervonic acid (C24:1, PubChem CID: 5281120)