Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish

Deng, Yun, Shanghai Jiao Tong University

Wang, Haihong, Shanghai Jiao Tong University

Liu, Xiaohui, Shanghai Jiao Tong University

Yuan, Hao, Shanghai Jiao Tong University

Xu, Jin, South China University of Technology

de Thé, Hugues, Hôpital Saint-Louis

Zhou, Jun, Shanghai Jiao Tong University, https://orcid.org/0000-0003-0472-3188

Zhu, Jun, Shanghai Jiao Tong University

zj10802@rjh.com.cn

Publication date: October 19, 2022

Publisher: Dryad

https://doi.org/10.5061/dryad.9cnp5hqms

Citation

Deng, Yun et al. (2022), Zbtb14 regulates monocyte and macrophage development through inhibiting pu.1 expression in zebrafish, Dryad, Dataset, https://doi.org/10.5061/dryad.9cnp5hqms

Abstract

To elucidate the mechanism underlying the aberrant monocyte/macrophage development of zbtb14^-/-mutant, GFP-positive cells were isolated from either wild-type Tg(mpeg1.1:eGFP) or zbtb14 ^-/- //Tg(mpeg1.1:eGFP) larvae at 2dpf by FACS. mRNA was extracted from sorted cells using RNeasy Micro (Qiagen, Manchester, UK) and mRNA libraries were constructed using NEBNext Ultra RNA Library Prep Kit for52 Illumina and sequenced under Illumina HiSeq X Ten with pair end 150bp (PE150).

Funding

National Natural Science Foundation of China, Award: 32171097