Heat-stress memory enhances acclimation of a migratory insect pest to global warming
Data files
Aug 13, 2024 version files 28.05 KB
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lxd1-1.csv
615 B
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lxd1-2.csv
1.85 KB
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lxd1-3.csv
1.64 KB
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lxd1-4.csv
2 KB
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lxd2-1.csv
705 B
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lxd2-2.csv
2.11 KB
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lxd2-3.csv
1.80 KB
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lxd2-4.csv
2.46 KB
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lxd3.csv
2.31 KB
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lxd4.csv
3.54 KB
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lxd5-1.csv
681 B
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lxd5-2.csv
1.29 KB
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lxd6.csv
2.38 KB
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README.md
4.68 KB
Abstract
In the face of rising global temperatures, the mechanisms behind an organism's ability to acclimate to heat stress remain enigmatic. The rice leaf folder, Cnaphalocrocis medinalis, traditionally viewed as temperature-sensitive, paradoxically exhibits robust larval acclimation to heat stress. This study used the heat-acclimated strain HA39, developed through multigenerational exposure to 39°C during the larval stage, and the unacclimated strain HA27 reared at 27°C to unravel the transgenerational effects of heat acclimation and its regulatory mechanisms. Heat acclimation for larvae incurred a fitness cost in pupae when exposed to high temperature, yet a significant transgenerational effect surfaced, revealing heightened fitness benefit in pupae from HA39, even without additional heat exposure during larval recovery at 27°C. This transgenerational effect exhibited a short-term memory, diminishing after two recovery generations. Moreover, the effect correlated with increased superoxide dismutase (SOD) enzyme activity and expression levels of oxidoreductase genes, representing physiological and molecular foundations of heat acclimation. Heat-acclimated larvae displayed elevated DNA methylation levels, while pupae from HA39, in recovery generations, exhibited decreased methylation indicated by the upregulation of a demethylase gene and downregulation of two methyltransferase genes at high temperature. In summary, heat acclimation induces DNA methylation, orchestrating heat-stress memory and influencing the expression levels of oxidoreductase genes and SOD activity. Heat-stress memory enhances acclimation of the migratory insect pest to global warming.
README: Heat-stress memory enhances acclimation of a migratory insect pest to global warming - DATA
Description of the data and file structure
6 datasets are present in this release related to the six main analyses of the manuscript. The columns description can be found in the analyses scripts. In short:
lxd1-1.csv: experimental data on survival rate of pupae exposured to different temperatures.
$ Exposed temperature: (num) Exposed temperature
$ Replicate: (num) No of the replicate for the experiment
$ Strain: (Chr) Strain of HA27 and HA39
$ Eclosion rate of pupae: (num) Eclosion rate of pupae
-lxd1-2.csv: experimental data on longevity of adult.
$ Exposed temperature: (num) Exposed temperature
$ Replicate: (num) No of the replicate for the experiment
$ Strain: (Chr) Strain of HA27 and HA39
$ Longevity: (num) Longevity of adult
-lxd1-3.csv: experimental data on fecundity of female.
$ Exposed temperature: (num) Exposed temperature
$ Replicate: (num) No of the replicate for the experiment
$ Strain: (Chr) Strain of HA27 and HA39
$ Fecundity: (num) Number of eggs per female
-lxd1-4.csv: experimental data on hatchability of eggs.
$ Exposed temperature: (num) Exposed temperature
$ Replicate: (num) No of the replicate for the experiment
$ Strain: (Chr) Strain of HA27 and HA39
$ Hatchability: (num) Hatchability of eggs
-lxd2-1.csv: experimental data measured to survival rate after recovery one generation.
$ Exposed temperature: (num) Exposed temperature
$ Replicate: (num) No of the replicate for the experiment
$ Strain: (Chr) Strain of HA27, HA39, and HA39-R1
$ Eclosion rate of pupae: (num) Eclosion rate of pupae
-lxd2-2.csv: experimental data measured to longevity of adult after recovery one generation.
$ Exposed temperature: (num) Exposed temperature
$ Replicate: (num) No of the replicate for the experiment
$ Strain: (Chr) Strain of HA27, HA39, and HA39-R1
$ Longevity: (num) Longevity of adult (d)
-lxd2-3.csv: experimental data measured to fecundity after recovery one generation.
$ Exposed temperature: (num) Exposed temperature
$ Replicate: (num) No of the replicate for the experiment
$ Strain: (Chr) Strain of HA27, HA39, and HA39-R1
$ Fecundity: (num) Number of eggs per female
-lxd2-4.csv: experimental data measured to Hatchability after recovery one generation.
$ Exposed temperature: (num) Exposed temperature
$ Replicate: (num) No of the replicate for the experiment
$ Strain: (Chr) Strain of HA27, HA39, and HA39-R1
$ Hatchability: (num) Hatchability of eggs
-lxd3.csv: experimental data measured to enzyme activity of SOD, CAT, and GST
$ Strain: (Chr) Strain of HA27 and HA39-R1
$ Replicate: (num) No of the replicate for the experiment
$ Exposed temperature: (num) Exposed temperature
$ SOD: (num) Enzyme activity of SOD
$ CAT: (num) Enzyme activity of CAT
$ GST: (num) Enzyme activity of GST
-lxd4.csv: experimental data measured to relative expression levels of CmGMC38 and CmGMC10 of HA27, HA39, HA27-R1, HA39-R1
$ Exposed temperature: (num) Exposed temperature
$ Replicate: (num) No of the replicate for the experiment
$ Strain: (Chr) Strain of HA27, HA39, and HA39-R1
$ CmGMC38: (num) Relative expression level of CmGMC38
$ CmGMC10: (num) Relative expression level of CmGMC10
-lxd5-1.csv: experimental data measured to DNA methylation level of larvae
$ Strain: (Chr) Strain of HA27, HA39
$ Replicate: (num) No of the replicate for the experiment
$ Exposed temperature: (num) Exposed temperature
$ Duration: (num) Duration of exposure (h)
$ DNA methylation levels: (Chr) DNA methylation levels in larvae
-lxd5-2.csv: experimental data measured to expression level (FPKM) of genes
$ epigenetic_gene_id: (Chr) Gene ID
$ HA27-27-1: (num) expression level in HA27 at 27oC sample 1
$ HA27-27-2: (num) expression level in HA27 at 27oC sample 2
$ HA27-27-3: (num) expression level in HA27 at 27oC sample 3
$ HA27-41-1: (num) expression level in HA27 at 41oC sample 1
$ HA27-41-2: (num) expression level in HA27 at 41oC sample 2
$ HA27-41-3: (num) expression level in HA27 at 41oC sample 3
$ HA39-41-1: (num) expression level in HA39 at 41oC sample 1
$ HA39-41-2: (num) expression level in HA39 at 41oC sample 2
$ HA39-41-3: (num) expression level in HA39 at 41oC sample 3
lxd6.csv: experimental data measured to relative expression levels of four genes
$ Strain: (Chr) Strain of larvae
$ Replicate: (num）Replicate for experiment
$ Exposed temperature: (chr) Exposed temperature
$ Cmed07565: (num) Relative expression level of Cmed07565
$ Cmed129345: (num) Relative expression level of Cmed12934
$ Cmed15355: (num) Relative expression level of Cmed15355
$ Cmed16639: (num) Relative expression level of Cmed16639