The research involved study of Lentil a nutritious and affordable historical plant. The research question was can the gene be classified according to the lentil and its relatives. The method involved using either phylogenetic or physiological characteristics of mesophilic bacteria isolated from a field-scale sludge composter were determined by 16S rDNA and phenotype analysis. The research used 34 mesophilic isolates of 5 (15%), 16 (47%) and 3 (9%) displayed amylase, protease activity. The result showed the binding proteins were stored in multigenes. The development was a resistance to environmental conditions. It concluded the information was known regarding microbial inhabitants of the ecosystem. In the study the 16S-DGGE approach estimated bacterial species and showed changes in the environment.