Data from: Glial ferritin maintains neural stem cells via transporting iron required for self-renewal in Drosophila
Data files
Aug 14, 2024 version files 5.50 MB
Abstract
Stem cell niche is critical for regulating the behavior of stem cells. Drosophila neural stem cells (Neuroblasts, NBs) are encased by glial niche cells closely, but it still remains unclear whether glial niche cells can regulate the self-renewal and differentiation of NBs. Here we show that ferritin produced by glia, cooperates with Zip13 to transport iron into NBs for the energy production, which is essential to the self-renewal and proliferation of NBs. The knockdown of glial ferritin encoding genes causes energy shortage in NBs via downregulating aconitase activity and NAD+ level, which leads to the low proliferation and premature differentiation of NBs mediated by Prospero entering nuclei. More importantly, ferritin is a potential target for tumor suppression. In addition, the level of glial ferritin production is affected by the status of NBs, establishing a bicellular iron homeostasis. In this study, we demonstrate that glial cells are indispensable to maintain the self-renewal of NBs, unveiling a novel role of the NB glial niche during brain development.
README: Glial ferritin maintains neural stem cells via transporting iron required for self-renewal in Drosophila
https://doi.org/10.5061/dryad.b5mkkwhnq
Description of the data and file structure
This dataset contains experimental data from a study aimed at investigating the neural stem cell function. The data includes measurements of neural stem cell number and proliferation, knockdown efficiency of ferritin, ferritin expression, ROS accumulation, ATP content, aconitase activity, NAD+ level, tumor size, and so on.
Files and variables
File: Source_Data.zip
Description: The Source_Data.zip supports Figures 1, 2, 3, 4, 5, 6, 7, Figure 1-Figure supplement 1,3, Figure 3-Figure supplement 1-3, Figure 4-figure supplement 1, Figure 5-figure supplement 1-3, Figure 6-figure supplement 1.
WB file contains original western blot results in Figure1F and 1H, Figure7A and 7B. RT-PCR file contains original DNA electrophoresis result in Figure 1D.
Access information
Other publicly accessible locations of the data:
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Data was derived from the following sources:
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Methods
This dataset contains experimental data from a study aimed at investigating the neural stem cell function. The data includes measurements of NB number and proliferation under different experimental conditions. The results of this study are discussed in detail in our relevant publication.