All original data used in the study published in the Journal of Visualized Experiments entitled: "Isolation of Targeted Hypothalamic Neurons for Studies of Hormonal, Metabolic, and Electrical Regulation" by Kathera-Ibarra-Forzisi et al. are provided in this dataset. 

Data are organized in sub-folders containing either confocal or bright-field images of primary hypothalamic neurons. The dataset does not contain analyses or measurements. For each figure of the paper, the data are organized in sub-folders corresponding to a specific experimental condition. The sub-folders are labeled with the corresponding figure number and the salient experimental conditions. For example, the sub-folder labeled "confocal images figure 3" contains all the original confocal images from which the representative images of the figure were chosen from. Likewise, the folder  "LepR+ figure 3" contains all the images of neurons immunolabeled with LepR antibody.  Data can be used by a third party for verification purposes but cannot be used for publication.

Abstract of the study: The hypothalamus regulates fundamental metabolic processes by controlling functions as varied as food intake, body temperature, and hormone release. As the functions of the hypothalamus are controlled by specific subsets of neuronal populations, the ability to isolate them provides a major tool for studying metabolic mechanisms. In this regard, the neuronal complexity of the hypothalamus poses exceptional challenges. For these reasons, new techniques, such as Magnetic-Activated Cell Sorting (MACS), have been explored. This paper describes a new application of magnetic-activated cell sorting (MACS) using microbead technology to isolate a targeted neuronal population from prenatal mice brains. The technique is simple and guarantees a highly pure and viable primary hypothalamic neuron culture with high reproducibility. The hypothalamus is gently dissociated, neurons are selectively isolated and separated from glial cells, and finally, using a specific antibody for a cell surface marker, the population of interest is selected. Once isolated, targeted neurons can be used to investigate their morphological, electrical, and endocrine characteristics and their responses in normal or pathological conditions. Furthermore, given the variegated roles of the hypothalamus in regulating feeding, metabolism, stress, sleep, and motivation, a closer look at targeted and region-specific neurons may provide insight into their tasks in this complex environment.

There are images of cells in culture taken under bright field or confocal microscopy.

Bright field images are in JPEG. confocal images are in Nikon ND2 format and can be imported in ImageJ or read through other freely available software.