Data for: Fabrication and characterization of antibacterial coatings using an amphoteric condensed tannin,Tanfloc
Data files
Nov 29, 2023 version files 300.32 KB
-
README.md
-
Tanfloc_PEM_dataset.zip
Abstract
This dataset presents comprehensive information on the development and characterization of antibacterial polyelectrolyte multilayer (PEM) coatings incorporating tanfloc, a plant-derived condensed tannin polymer with inherent antimicrobial properties. The dataset encompasses experimental data related to the fabrication of PEMs using tanfloc as either a polyanion or a polycation, expanding the versatility of this amphoteric polymer in PEM coatings. Typically, PEMs are formed by combining a polycation and a polyanion with complementary ionic groups. However, the unique amphoteric nature of tanfloc allows for the creation of PEMs utilizing only one of its functional groups, leaving the other functional group available for imparting antibacterial activity.
The dataset includes details on the assembly of tanfloc-containing PEMs employing various counter-polyelectrolytes, including three poly-anionic glycosaminoglycans with varying charge densities, as well as the polycations N,N,N-trimethyl chitosan and polyethylenimine. The layer-by-layer assembly of PEMs is monitored using in situ Fourier-transform surface plasmon resonance (FT-SPR), confirming stable layer-by-layer construction. Surface chemistry is assessed through X-ray photoelectron spectroscopy (XPS).
Furthermore, this dataset provides insights into the biocompatibility of tanfloc-containing PEMs, demonstrating their support for mammalian cells. Most notably, the dataset includes extensive data on the antiadhesive and antibacterial properties of these coatings against common implant-associated pathogens, such as Staphylococcus aureus and Pseudomonas aeruginosa. The antibacterial effects observed are attributed to electrostatic interactions and the polyphenolic nature of tanfloc.
README: Dataset for Fabrication and Characterization of Antibacterial Coatings using an Amphoteric Condensed Tannin, Tanfloc.
https://doi.org/10.5061/dryad.gqnk98sv9
The dataset contains experimental data detailing the development and characterization of antibacterial polyelectrolyte multilayer (PEM) coatings containing tanfloc, a natural condensed tannin polymer, as an amphoteric biomaterial. It includes results from biocompatibility assessments using human adipose-derived stem cells (ADSCs) and antibacterial activity assays against Pseudomonas aeruginosa and Staphylococcus aureus. Moreover, the dataset provides insights into tanfloc hydrodynamic properties, the real-time assembly of PEMs via Fourier-Transform Surface Plasmon Resonance (FT-SPR), and PEM compositional analysis by X-ray Photoelectron Spectroscopy (XPS). N,N,N-Trimethyl chitosan is also characterized via 1H Nuclear Magnetic Resonance (1H NMR) spectroscopy. Additionally, fluorescence microscopy data quantifies live and dead bacterial adhesion, demonstrating the antibacterial efficacy of the coatings. These diverse datasets are valuable for research in biomaterials and antimicrobial coatings.
Description of the data and file structure
We've organized the data into seven main folders, each containing valuable information related to various experiments and analyses.
Folder Structure
1. H NMR
Content: This folder contains 1H Nuclear Magnetic Resonance (NMR) spectra, to confirm the synthesis of N,N,N-trimethyl chitosan.
File Format: Spectra data is stored in .csv files.
File Name: TMC H_NMR.csv
2. DLS
Content: In this folder, you'll find data related to the hydrodynamic diameter and polydispersity index of tanfloc solutions at different pH values (5, 6, 7.4, 8.4, and 9.3) collected using Dynamic Light Scattering (DLS).
File Format: Data is provided in .txt format.
File Name: DLS_TAN.txt
3. FT_SPR
Content: Here, you'll discover real-time monitoring data for the deposition of Polyelectrolyte Multilayers (PEMs) using Fourier-Transform Surface Plasmon Resonance (FT-SPR).
File Format: Data is stored in .csv files.
File Name: FT_SPR.csv
4. XPS
Content: The X-ray Photoelectron Spectroscopy (XPS) folder is divided into two subfolders:
4.1. Survey:
Content: Contains XPS survey spectra related to various PEMs (TAN-HEP, TAN-HA, TAN-CS, TMC-TAN, and PEI-TAN), each assessed in 12 and 13 layers.
File Format: Data is stored in .csv files.
File Names: Survey.csv
4.2. High Resolution:
Content: Contains detailed high-resolution spectra for carbon (C1s), nitrogen (N1), oxygen (O1), and sulfur (S2p) envelopes of the same PEMs.
Subfolders: There are ten subfolders corresponding to different PEMs, each with two layers (12 and 13).
TAN_HEP12
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
S2p.csv
TAN_HEP13
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
S2p.csv
TAN_HA12
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
TAN_HA13
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
TAN_CS12
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
S2p.csv
TAN_CS13
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
S2p.csv
TMC_TAN12
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
TMC_TAN13
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
PEI_TAN12
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
PEI_TAN13
File Format: Data is stored in .csv files.
File Names:
C1.csv
N1.csv
O1.csv
5. LDH
Content: Lactate Dehydrogenase Assay (LDH) data related to absorbance at 490 nm and 680 nm, which indicates cytotoxicity.
File Format: Data is provided in .csv format.
File Name: LDH.csv
6. Bacterial Growth Inhibition
Content: This folder is further divided into two subfolders:
6.1. Staphylococcus aureus
6.2. Pseudomonas aeruginosa
Each subfolder contains data files related to absorbance at 560 nm using a plate reader, indicating bacterial growth inhibition at two different time points (6 and 24 hours).
File Format: Data is stored in .csv files.
File Names:
bacteria_inhibition_6hr.csv
bacteria_inhibition_24hr.csv
7. Live-Dead Bacterial Cell
Content: Similar to the Bacterial Growth Inhibition folder, this folder is also divided into two subfolders:
7.1. Staphylococcus aureus
7.2. Pseudomonas aeruginosa
Each subfolder contains data files related to live and dead bacterial percentages determined using ImageJ software at two different time points (6 and 24 hours).
File Format: Data is stored in .csv files.
File Names:
6hr.csv
24hr.csv
Note:
1. FT_SPR Data Explanation:
The 'FT_SPR' data sheet contains numerical values reflecting measurements obtained from specific software during the designated period. These values vary across different samples due to variations in sample properties and measurement conditions. It is important to note that null values in this data sheet correspond to cells in the Excel file that were not reported by the software. These null values indicate a lack of reported data for those specific cells.
2. Bacterial Growth Inhibition Data:
The 'Bacterial Growth Inhibition' data is quantified using absorbance values, which are obtained through a plate reader. This instrument measures the absorbance of solutions placed in a 96-well plate. It is essential to understand that empty cells in this dataset should not be filled by users. These empty cells represent instances where measurements were either not applicable or not obtained, and thus should be left as is to maintain the integrity of the dataset.
3. Live-Dead Bacterial Cell Data:
For the 'Live-Dead Bacterial Cell' data, an effort was made to measure the number of cells in at least five different areas for each sample. Depending on the sample characteristics, this measurement could either be limited to exactly five points or include more. To maintain clarity and consistency in our dataset, empty cells, where measurements were not possible or not taken, have been filled with null values by the authors.
Methods
1H Nuclear Magnetic Resonance (1H NMR): Tanfloc (TAN) was characterized using 1H NMR spectroscopy, recording spectra on a Bruker Neo US 400 NMR spectrometer operating at 400 MHz.
Dynamic Light Scattering (DLS): Solutions of TAN (tanfloc) with varying pH values (5, 6, 7.4, 8.4, and 9.3) were prepared and subjected to dynamic light scattering (DLS) to determine the hydrodynamic diameter and polydispersity index (PDI). This characterization was performed using a Zetasizer Nano ZS instrument from Malvern.
Fourier-Transform Surface Plasmon Resonance (FT-SPR): The layer-by-layer (LBL) assembly of PEMs on gold-coated glass chips was monitored in real-time using Fourier-Transform Surface Plasmon Resonance (FT-SPR). This involved modifying the gold-coated chips, flowing polycation, polyanion, and rinse solutions, and collecting data using an SPR-100 module coupled to a Nicolet 8700 FT-IR spectrometer.
X-ray Photoelectron Spectroscopy (XPS): Composition analysis of the PEMs was performed using X-ray Photoelectron Spectroscopy (XPS). A survey scan was conducted over the energy range of 10 to 1100 eV, with high-resolution spectra obtained for the carbon (C1s), nitrogen (N1s), oxygen (O1s), and sulfur (S2p) envelopes.
LDH Cytotoxicity Assay (ADSC cells): Human adipose-derived stem cells (ADSCs) at passage 6 were cultured in Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum and 1.0% penicillin/streptomycin. Sterilized samples were prepared and cells were seeded onto them. After 24 hours, culture media from each well were collected and subjected to the lactate dehydrogenase (LDH) cytotoxicity assay. This involved measuring absorbance at 490 nm and 680 nm using a plate reader (BMG LABTECH FLUOstar Omega). Control samples on polystyrene (PS) were included for comparison.
Bacterial Growth Inhibition Assay: The antibacterial activity of different PEMs was assessed against both Gram-negative Pseudomonas aeruginosa (P. aeruginosa) and Gram-positive Staphylococcus aureus (S. aureus). Bacteria were cultured in nutrient broth media (NBM) and diluted to a concentration of 106 CFU/mL. Subsequently, 500 μL of the bacterial solution was exposed to the surfaces for 6 and 24 hours. After incubation, a 200 μL aliquot of the solution was extracted, and absorbance at 560 nm was measured using a plate reader. Control samples of glass and polystyrene (PS) were also included under identical conditions.
Live-Dead Bacterial Cell Quantification: After incubation for 6 and 24 hours, bacterial adhesion to surfaces was visualized using fluorescence microscopy. The surfaces were stained and fixed before analysis. Live and dead bacterial percentages were determined using ImageJ software (n = 3).