Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera

Feau, Nicolas, University of British Columbia, https://orcid.org/0000-0001-5925-9867

Wong, Barbara, University of British Columbia

Hamelin, Richard, University of British Columbia

feaunico@mail.ubc.ca, barbaraawong@gmail.com, Richard.Hamelin@ubc.ca

Published Jan 07, 2020 on Dryad. https://doi.org/10.5061/dryad.jq2bvq861

Cite this dataset

Feau, Nicolas; Wong, Barbara; Hamelin, Richard (2020). Molecular assays to detect the presence and viability of Phytophthora ramorum and Grosmannia clavigera [Dataset]. Dryad. https://doi.org/10.5061/dryad.jq2bvq861

Abstract

Protocols for the RNA and DNA extractions, cDNA synthesis and qPCR and RT-qPCR for detecting and assessing viability of the Phytophthora ramorum and Grosmannia clavigera tree pathogens.

Methods

Fifty to 80 mg of wood scrapings were collected at each inoculation point from eight logs of four conifer species and used for the simultaneous extractions of gDNA and mRNA.

Using the diluted RNA, cDNA, synthesis was performed using the QuantiTect Reverse Transcription Kit (QIAGEN).