NGS Data from: Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage

Riesenberg, Stephan, Max Planck Institute for Evolutionary Anthropology, https://orcid.org/0000-0001-8171-1717

Helmbrecht, Nelly, Max Planck Institute for Evolutionary Anthropology

Kanis, Philipp, Max Planck Institute for Evolutionary Anthropology

Maricic, Tomislav, Max Planck Institute for Evolutionary Anthropology

Pääbo, Svante, Max Planck Institute for Evolutionary Anthropology

stephan_riesenberg@eva.mpg.de

Published Jan 19, 2022 on Dryad. https://doi.org/10.5061/dryad.jwstqjqb7

Cite this dataset

Riesenberg, Stephan et al. (2022). NGS Data from: Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage [Dataset]. Dryad. https://doi.org/10.5061/dryad.jwstqjqb7

Abstract

We engineered gRNAs with highly stable hairpins in their constant parts and further enhanced their stability by chemical modifications. The ‘Genome-editing Optimized Locked Design’ (GOLD)-gRNA increases genome editing efficiency up to around 1000-fold (from 0.08% to 80.5%) with a mean increase across different other targets of 7.4-fold. The related NGS data is deposited here.

Funding

Max Planck Society

Nomis Foundation