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Data from: Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue

Citation

Morlan, John D.; Qu, Kunbin; Sinicropi, Dominick V. (2012), Data from: Selective depletion of rRNA enables whole transcriptome profiling of archival fixed tissue, Dryad, Dataset, https://doi.org/10.5061/dryad.k83b8

Abstract

We report a method for Selective Depletion of abundant RNA (SDRNA) species from Human total RNA isolated from formalin-fixed, paraffin-embedded (FFPE) tissue, here demonstrating removal of ribosomal and mitochondrial transcripts from clinical FFPE tissue RNA archived up to 20 years. Importantly, SDRNA removes 98% of targeted RNAs while preserving relative abundance profiles of non-targeted RNAs, enabling routine whole transcriptome analysis of clinically valuable archival tissue specimens by Next-Generation Sequencing.

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