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Data from: A long PCR based approach for DNA enrichment prior to next-generation sequencing for systematic studies

Citation

Uribe-Convers, Simon; Duke, Justin R.; Moore, Michael J.; Tank, David C. (2014), Data from: A long PCR based approach for DNA enrichment prior to next-generation sequencing for systematic studies, Dryad, Dataset, https://doi.org/10.5061/dryad.kc75n

Abstract

Premise of the study: We present an alternative approach for molecular systematic studies that combines long PCR and next-generation sequencing (NGS). Our approach can be used to generate templates from any DNA source for NGS. Here we test our approach by amplifying complete chloroplast genomes and we present a set of 58 potentially universal primers for angiosperms to do so. Additionally, this approach is likely to be particularly useful for nuclear regions. Methods and Results: Chloroplast genomes of 30 species across angiosperms were amplified to test our approach. Amplification success varied depending on whether PCR conditions were optimized for a given taxon. To further test our approach, some amplicons were sequenced on an Illumina HiSeq 2000. Conclusions: Although here we tested this approach by sequencing plastomes, long PCR amplicons could be generated using DNA from any genome, expanding the possibilities of this approach for molecular systematic studies.

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