Phylogenetics identifies two eumetazoan TRPM clades and an eighth TRP family, TRP soromelastatin (TRPS)
Cite this dataset
Himmel, Nathaniel J; Gray, Thomas R; Cox, Daniel N (2020). Phylogenetics identifies two eumetazoan TRPM clades and an eighth TRP family, TRP soromelastatin (TRPS) [Dataset]. Dryad. https://doi.org/10.5061/dryad.kwh70rz03
Abstract
Transient receptor potential melastatins (TRPMs) are most well known as cold and menthol sensors, but are in fact broadly critical for life, from ion homeostasis to reproduction. Yet, the evolutionary relationship between TRPM channels remains largely unresolved, particularly with respect to the placement of several highly divergent members. To characterize the evolution of TRPM and like channels, we performed a large-scale phylogenetic analysis of >1,300 TRPM-like sequences from 14 phyla (Annelida, Arthropoda, Brachiopoda, Chordata, Cnidaria, Echinodermata, Hemichordata, Mollusca, Nematoda, Nemertea, Phoronida, Priapulida, Tardigrada, and Xenacoelomorpha), including sequences from a variety of recently sequenced genomes that fill what would otherwise be substantial taxonomic gaps. These findings suggest: 1) the previously recognized TRPM family is in fact two distinct families, including canonical TRPM channels and an eighth major previously undescribed family of animal TRP channel, TRP soromelastatin; 2) two TRPM clades predate the last bilaterian–cnidarian ancestor; and 3) the vertebrate–centric trend of categorizing TRPM channels as 1–8 is inappropriate for most phyla, including other chordates.
Methods
Searches for TRPS (ced-11-like), TRPN, and TRPC sequences followed the same protocol. For TRPS, sequences from Caenorhabditis elegans, Strigamia maritima, and Octopus vulgaris were used as bait. For TRPN and TRPC datasets, Drosophila melanogaster nompC (isoform PA, FlyBase ID: FBpp0084879) and Trp (isoform PA, Flybase ID: FBpp0084879) served as bait sequences, respectively.
Funding
National Institute of Neurological Disorders and Stroke, Award: R01NS115209
National Institute of General Medical Sciences, Award: R25GM109442-01A1