Gene expression is controlled at multiple levels to ensure that the gene product is present at the right place, time, and quantity during development or in response to environmental stress. Transcriptional and post-transcriptional regulation of RNA abundance, processing, and translation control protein abundance by gene-specific and genome-wide mechanisms. Circular RNAs (circRNAs) have been identified as a new group of multifunctional gene-specific regulators. CircRNAs have been identified in over 20 plant species to date and although they appear to be highly conserved across the plant kingdom, sequencing of circRNA has not been performed for any oil seed crop. Among the many high-value oilseed crops, Camelina sativa has gained much attention for its potential use as a model species for studying oil biosynthesis. This is partly due to its close ancestral relationship to the well-established model species Arabidopsis thaliana, a system that has been especially useful in studying the mechanisms and functions of various circRNAs. As a recently diverged allohexaploid, Camelina sativa may be subject to even more complexity in regulation due to retention of multiple, highly-similar subgenomes with redundant gene copies. In this study, we performed deep sequencing in Camelina seedlings germinated under light or dark conditions to identify circRNA and examine correlated expression patterns of linear RNA cognates under different light regimes during seedling establishment.

Data are unprocessed 150 bp paired-end FASTQ reads generated from the sequencing run. FASTQ files were demultiplexed from the original base call files using Illumina's FASTQ generator analysis pipeline within Local Run Manager. 

Files labeled "5D" correspond to dark-treated seedlings harvested after 5 days of germination, as indicated in the README.txt file.

Files labeled "7D" correspond to dark-treated seedlings harvested after 7 days of germination, as indicated in the README.txt file.

Files labeled "5L" correspond to light-treated seedlings harvested after 5 days of germination, as indicated in the README.txt file.

Files labeled "7L" correspond to light-treated seedlings harvested after 7 days of germination, as indicated in the README.txt file.