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Data from: A field study evaluating the humoral immune response in Mongolian sheep vaccinated against sheeppox virus

Citation

Limon, Georgina et al. (2021), Data from: A field study evaluating the humoral immune response in Mongolian sheep vaccinated against sheeppox virus, Dryad, Dataset, https://doi.org/10.5061/dryad.msbcc2fvx

Abstract

Sheeppox is a transboundary disease of sheep caused by infection with the capripoxvirus sheeppox virus (SPPV). Sheeppox is found in Africa, the Middle East and Asia and is characterised by fever, multifocal cutaneous raised lesions, and death, with substantial negative impact on affected flocks. Vaccination with live attenuated capripoxvirus (CPPV) strains is an effective and widely used means of controlling sheeppox outbreaks, however there are few reports of post-vaccination field surveillance studies of sheeppox. This study used a commercially available ELISA and a fluorescence-based neutralisation assay (FVNT) to examine quantitative and temporal features of the humoral response of sheep vaccinated with a live attenuated CPPV strain in Mongolia. 400 samples were tested using the ELISA, and a subset of 45 also tested with the FVNT. There was substantial agreement between the FVNT and ELISA tests. Antibodies to CPPV were detected between 40 and 262 days post vaccination. There was no significant difference between serological status (positive / negative) and sex or age, however an inverse correlation was found between the length of time since vaccination and serological status. Animals between 90 and 180 days post-vaccination were more likely to be positive than animals greater than 180 days post vaccination. This data provides temporal parameters to consider when planning sheeppox post-vaccination monitoring programmes. In summary, our results show a commercial CPPV ELISA kit is a robust and reliable assay for use in resource-restricted low and low-middle income countries for post CPPV vaccination surveillance on a regional or national level.

Methods

Blood samples from sheep and associated data were collected as part of the post-vaccination surveillance programme for sheeppox implemented by the Mongolian General Authority for Veterinary Services (GAVS) in 2016.

Usage Notes

The attached .xls file contains all raw data used in the associated publication, " A comparative serological field study evaluating the humoral immune response in Mongolian sheep vaccinated against sheeppox virus." The dataset identifies all individual sheep by a unique identification number (ID_num). Each unique ID_num is associated with relevant metadata: Province name, Sum name, herder name coded (Herded_Id), animal species, age of the animal, in years, when sampled (Age), sex of the animal “F” or “M” (Sex), date when the animal was sampled (Date_sample_collected), date when the animal was vaccinated for sheeppox according to the vaccination records (Date_vaccinated_raw data), and for samples in which the exact date of vaccination was not available and a range of potential time was given, the midpoint date within this range (Date_vaccinated_midpoint), animals for which vaccination date was not available receive an "NA" value; time from vaccination to sampled in days (Time_from_vaccinated_midpoint);  %S/P values for the ELISA test conducted in the Mongolia lab (Ag_ELISA_SCVL_OD_SP) and %S/P values for the ELISA test conducted in the UK lab (Ag_ELISA_TPI_OD_SP), results from the ELISA test classified as binary variable “Positive” or “Negative” (Ag_ELISA_SCVL_bin and Ag_ELISA_TPI_bin); titres from the fluorescence-based neutralisation assay (FVNT Titre) and results from the FVNT test classified as binary variable (“Positive” or “Negative”), all animals that were not tested by FVNT receive an "NA" value in these columns.

Funding

Biotechnology and Biological Sciences Research Council, Award: BBS/E/I/00007031

Biotechnology and Biological Sciences Research Council, Award: BB/E/I/00007036

Biotechnology and Biological Sciences Research Council, Award: BB/E/I/00007037

Biotechnology and Biological Sciences Research Council, Award: BBS/E/I/00007039

Biotechnology and Biological Sciences Research Council, Award: BB/J004324/1

Biotechnology and Biological Sciences Research Council, Award: BBS/E/D/20002173

Horizon 2020, Award: 773701

Food and Agriculture Organization of the United Nations, Award: TCP/MON/3603

IdVet

Food and Agriculture Organization of the United Nations, Award: TCP/MON/3603

IdVet