Tracking an animal's location from video has many applications, from providing information on health and welfare to validating sensor-based technologies. Typically, accurate location estimation from video is achieved using cameras with overhead (top-down) views, but structural and financial limitations may require mounting cameras at other angles. We describe a user-friendly solution to manually extract an animal's location from non-overhead video. Our method uses QGIS, an open-source geographic information system, to: (1) assign facility-based coordinates to pixel coordinates in non-overhead frames; 2) use the referenced coordinates to transform the non-overhead frames to an overhead view; and 3) determine facility-based x, y coordinates of animals from the transformed frames. Using this method, we could determine an object's facility-based x, y coordinates with an accuracy of 0.13 ± 0.09 m (mean ± SD; range: 0.01–0.47 m) when compared to the ground truth (coordinates manually recorded with a laser tape measurer). We demonstrate how this method can be used to answer research questions about space-use behaviors in captive animals, using 6 ewe-lamb pairs housed in a group pen. As predicted, we found that lambs maintained closer proximity to their dam compared to other ewes in the group and lamb-dam range sizes were strongly correlated. However, the distance traveled by lambs and their dams did not correlate, suggesting that activity levels differed within the pair. This method demonstrates how user-friendly, open-source GIS tools can be used to accurately estimate animal location and derive space-use behaviors from non-overhead video frames. This method will expand capacity to obtain spatial data from animals in facilities where it is not possible to mount cameras overhead.

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