Data from: Phenolics lie at the center of functional versatility in the responses of two phytochemically diverse tropical trees to canopy thinning
Schneider, Gerald F.
Coley, Phyllis D.
Younkin, Gordon C.
Forrister, Dale L.
Mills, Anthony G.
Kursar, Thomas A.
Published Sep 16, 2020 on Dryad.
Cite this dataset
Schneider, Gerald F. et al. (2020). Data from: Phenolics lie at the center of functional versatility in the responses of two phytochemically diverse tropical trees to canopy thinning [Dataset]. Dryad. https://doi.org/10.5061/dryad.t469v60
Saplings in the shade of the tropical understorey face the challenge of acquiring sufficient carbon for growth as well as defence against intense pest pressure. A minor increase in light availability via canopy thinning may allow for increased investment in chemical defence against pests, but it may also necessitate additional biochemical investment to prevent light-induced oxidative stress. The shifts in secondary metabolite composition that increased sun exposure may precipitate in such tree species present an ideal milieu for evaluating the potential of a single suite of phenolic secondary metabolites to be used in mitigating both abiotic and biotic stressors. To conduct such an evaluation, we exposed saplings of two unrelated species to a range of light environments and compared changes in their foliar secondary metabolome alongside corresponding changes in the abiotic and biotic activity of their secondary metabolite suites. Among the numerous classes of secondary metabolites found in both species, phenolics accounted for the majority of increases in antioxidant and UV-absorbing properties as well as activity against an invertebrate herbivore and a fungal pathogen. Our results support the hypothesis that phenolics contribute to the capacity of plants to resist co-occurring abiotic and biotic stressors in resource-limited conditions.
Data from FRAP/FRASC assays for primary and secondary metabolites.
Protocol for FRAP and FRASC antioxidant assays.
Data for Artemia franciscana assay of secondary metabolite extracts from Alseis blackiana and Brosimum utile.
Gravimetric data for all extract fractions from Alseis blackiana and Brosimum utile.
Data from assay of secondary metabolite extracts from Alseis blackiana and Brosimum utile with a Glomerellaceae fungal pathogen.
Protocol for assay of secondary metabolite extracts from Alseis blackiana and Brosimum utile with Glomerellaceae fungal pathogen.
Photosynthetic flux, temperature, and relative humidity data for the uniform shade treatments.
UV irradiance data for experimental shadehouse site.
Photosynthetic flux data for sunflecks treatment.
UV absorbance of UPLC-MS features in secondary metabolite extracts of Alseis blackiana and Brosimum utile.
Sample number-treatment data for all samples of Alseis blackiana and Brosimum utile.
R code for extracting UV absorbance data from Waters UPLC-MS data.
R code for processing and analysis of UPLC-MS data with XCMS and MetaboAnalyst.
Detailed results of statistical analyses of secondary metabolite fold-change across treatments
Individual compound class and fold-change
Classification and fold-change data for each compound in both species and leaf ages
Brosimum utile molecular network
Molecular network visualisation file for Brosimum utile. For use with Cytoscape.
Alseis blackiana molecular network
Molecular network visualisation file for Alseis blackiana. For use with Cytoscape.
National Science Foundation, Award: DEB-1405637, DEB-1135733