MoS₂–Ti_L/MoS₂–Ti_H multilayered coatings (where L and H represent low- and high-powered of the sputtering titanium target) exhibit excellent mechanical and tribological performance owing to their multilayered microstructure. Herein, the effect of two different modulation periods on the mechanical and tribological performance of the multilayered coatings was studied. The performance of the coatings were found to depend on modulation periods of single layer thickness and thickness ratio respectively. When the thickness ratio of MoS₂–Ti_L layer to MoS₂–Ti_H layer is fixed with different number of layers, the adverse effects of the interface outweigh the beneficial effect; thus, the mechanical and tribological performance of the multilayered coatings are improved with an increase in the single layer thickness. When the effect of the multilayer interfaces on the studied coatings is similar with the same number of layers, the MoS₂–Ti_H layer has more impact on the hardness of the MoS₂–Ti_L/MoS₂–Ti_H multilayered coatings, whereas the MoS₂–Ti_L layer substantially affects the friction and wear resistance.