Endoscopic and histopathological findings in dyspeptic patients with Helicobacter pylori infection

Name: Endoscopic and histopathological findings in dyspeptic patients with Helicobacter pylori infection
Creator: Ajaya Basnet

Basnet, Ajaya1

Published Jan 16, 2024 on Dryad. https://doi.org/10.5061/dryad.x0k6djhs6

Data files

Jan 16, 2024 version files 24.21 KB

Dataset.xlsx

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README.md

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Abstract

Background: Despite advancements in medicine, gastrointestinal diseases caused by Helicobacter pylori continue to be common in developing countries. This study analyzed gastric mucosal biopsies from the Manipal College of Medical Sciences in Pokhara, Nepal, between January 2017 and June 2018 to determine the prevalence of H. pylori, its association with histopathological changes, and evaluate it using OLGA and OLGIM systems.

Methods: In this analytical cross-sectional study, 80 patients with dyspepsia were included. Biopsies were collected, stored, and labeled. The biopsies were fixed, cut into sections, and stained with routine H&E and Giemsa stains. Evaluations were made using OLGA and OLGIM staging. Statistical analyses were performed using SPSS version 17.0, with significance determined at p<0.05.

Results: Hospital-visiting patients had an 8.41% (38/452) [10.97–5.85%, 95% Confidence Interval (CI)] rate of H. pylori infection, while dyspeptic patients had 47.50% (38/80) (58.44–36.56, 95% CI). Females (48%, 12/25) and patients aged 31–40 (62.50%, 5/8) were mostly affected. The most common clinical presentation and endoscopic findings relating to infection included weight loss (15.79%) and erythema (44.74%), respectively. Provisional and histopathological diagnoses of infected patients mostly included gastritis (50%) and chronic gastritis (63.16%), respectively. Hundred percent of moderate H. pylori infections were associated with normal atrophy and intestinal metaplasia, while 89.66% of mild infections were associated with mild active and chronic inflammation. OLGIM downgraded one OLGA stage 2 and 4 and two OLGA stage 3 cases.

Conclusion: H. pylori infections were most common in adults and females with chronic gastritis. OLGIM was less accurate than OLGA in evaluating high-risk cases, such as adenocarcinomas.

README: Endoscopic and histopathological findings in dyspeptic patients with Helicobacter pylori infection

https://doi.org/10.5061/dryad.x0k6djhs6

The dataset is of dyspeptic patients with and without Helicobacter pylori infection. Dataset comprises a single sheet. The sheet details for demographic information, such as age group and gender of the dyspeptic patients; clinical manifestations, including upper gastrointestinal bleeding, abdominal pain, anorexia, weight loss; endoscopic findings comprising erythema, ulcer, atrophy, growth; provisional endoscopic diagnoses, comprising gastritis, benign gastric ulcer, malignancy; histopathological diagnoses, corpus and antrum abnormalities, and Operative Link on Gastritis Assessment and Operative Link on Gastric Intestinal Metaplasia scoring and staging.

Exact or direct age is removed and is categorized as age group to anonymize the data.

Data were anonymized with the code. Patients with dyspepsia were coded with alphanumeric number with initial P i.e. Pno.

Units of the study variables were standard and as follows:

(a) Age group Years

An oral lignocaine spray was administered before the procedure for a local anesthetic effect. The surgeon performed gastric mucosal biopsies after endoscopic evaluation. Hematoxylin and eosin-stained sections were examined under a microscope to identify various gastric mucosal histopathological changes. The modified Giemsa stain was utilized to examine all sections for the presence of H. pylori. The stained sections were graded according to the Updated Sydney System,24 OLGA System, and OLGIM System.

Except for age, the data were qualitative and were calculated as frequency (percentage) in SPSS version 17.0. Quantitative variables were calculated as median (interquartile range) and were analyzed using t-test for statistical correlations, while qualitative variables were calculated as number (percentage) and tested using chi-square test at 95% confidence interval.

S.N. represents serial number of patients, 0 represents female, 1 represents male, OLGA represents Operative Link on Gastritis Assessment, and OLGIM represents Operative Link on Gastric Intestinal Metaplasia

Readers may access the data from the Dryad repository or with a request email to the corresponding author, Ajaya Basnet (abasnet.microberesearch@gmail.com), of the article.