Fast-spiking interneuron detonation drives high-fidelity inhibition in the olfactory bulb
Data files
Aug 06, 2024 version files 39.47 MB
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Fig1B.txt
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Fig1C.txt
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Fig1D.txt
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Fig1E.txt
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Fig1F.xls
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Fig1Hinset.txt
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Fig1Jinset.txt
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Fig1K.xls
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Fig1Lleft.txt
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Fig1Lright.txt
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Fig1Mleft.txt
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Fig1Mright.txt
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Fig1Nleft.txt
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Fig1Nright.txt
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Fig1Oleft.txt
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Fig1Oright.txt
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Fig1PQRS.xls
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Fig2CD.xls
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Fig3B.txt
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Fig3C.txt
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Fig3D.txt
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Fig3E.txt
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Fig3F.txt
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Fig3GH.xls
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Fig4Bleft.txt
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Fig4Bright.txt
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Fig4Dleft.txt
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Fig4Dright.txt
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Fig4EGJ.xls
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Fig4H.txt
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Fig4K.txt
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Fig5Bleft.txt
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Fig5Bright.txt
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Fig5Cleft.txt
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Fig5Cright.txt
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Fig5Eleft.txt
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Fig5Eright.txt
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Fig5Fleft.txt
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Fig5Fright.txt
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Fig5GH.xls
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Fig6CFG.xls
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Fig7B.txt
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Fig7C.txt
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Fig7D_FSI.txt
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Fig7D_total.txt
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Fig7D.xls
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README.md
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S10Fig.xls
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S11Fig.xls
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S13FigB.txt
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S13FigC.txt
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S13FigE.txt
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S13FigF.txt
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S14Fig.xls
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S1Fig.xls
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S1Table.xls
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S2FigAFKP.txt
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S2FigAFKPinset.txt
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S2FigB.txt
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S2FigBfit.txt
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S2FigCHMR.txt
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S2FigDEIJNOST.xls
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S2FigG.txt
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S2FigGfit.txt
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S2FigL.txt
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S2FigLfit.txt
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S2FigQ.txt
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S2FigQfit.txt
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S2Table.xlsx
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S6FigA.txt
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S6FigBleft.txt
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S6FigBright.txt
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S6FigC.xls
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S7Fig.xls
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S8FigACDF.xlsx
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S8FigB_ctrl.txt
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S8FigB_NA.txt
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S8FigB_NAG.txt
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S8FigB_wash.txt
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S8FigE_ctrl.txt
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S8FigE_NA.txt
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S8FigE_NAG.txt
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S8FigE_wash.txt
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S9FigB.txt
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S9FigCleft.txt
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S9FigCright.txt
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S9FigE.txt
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S9FigFleft.txt
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S9FigFright.txt
Abstract
Inhibitory circuits in the mammalian olfactory bulb (OB) dynamically reformat olfactory information as it propagates from peripheral receptors to the downstream cortex. To gain mechanistic insight into how specific OB interneuron types support this sensory processing, we examine unitary synaptic interactions between excitatory mitral and tufted cells (MTCs), the OB projection neurons and a conserved population of anaxonic external plexiform layer interneurons (EPL-INs) using pair and quartet whole-cell recordings in acute mouse brain slices. Physiological, morphological, neurochemical, and synaptic analyses divide EPL-INs into distinct subtypes and reveal that parvalbumin-expressing fast-spiking EPL-INs (FSIs) perisomatically innervate MTCs with release-competent dendrites and synaptically detonate to mediate fast, short-latency recurrent and lateral inhibition. Sparse MTC synchronization supralinearly increases this high-fidelity inhibition, while sensory afferent activation combined with single-cell silencing reveals that individual FSIs account for a substantial fraction of total network-driven MTC lateral inhibition. OB output is thus powerfully shaped by detonation-driven high-fidelity perisomatic inhibition.
README: Fast-spiking interneuron detonation drives high-fidelity inhibition in the olfactory bulb
https://doi.org/10.5061/dryad.xd2547dr6
"NaN" = "Not a Number", used to indicate entries for which no data is available (e.g., if a neuron's physiological data was successfully recorded but its morphology was not recovered in the post hoc staining process, then morphological measurements for that neuron are listed as "NaN").
Fig 1
- Panels B-E
- Fig1B.txt, Fig1C.txt, Fig1D.txt, Fig1E.txt
- column 1: time (s): column 2: low current (pA); column 3: low voltage (mV); column 4: high current (pA); column 5: high voltage (mV)
- Panel F
- Fig1F.xls
- Panels H inset, J inset
- Fig1Hinset.txt, Fig1Jinset.txt
- column 1: time (ms): column 2: low current (pA); column 3: low voltage (mV); column 4: high current (pA); column 5: high voltage (mV)
- Panel K
- Fig1K.xls
- Panels L-O
- Fig1Lleft.txt, Fig1Lright.txt, Fig1Mleft.txt, Fig1Mright.txt, Fig1Nleft.txt, Fig1Nright.txt, Fig1Oleft.txt, Fig1Oright.txt
- column 1: time (ms); column 2: mean voltage (mV) or current (pA); columns 3-end: individual trials
- Panels P-S
- Fig1PQRS.xls
Fig 2
- Panels C, D
- Fig2CD.xls
Fig 3
- Panel B
- Fig3B.txt
- column 1: time (s); column 2: FSI current (nA); column 3: MTC current (nA)
- Panel C
- Fig3C.txt
- column 1: delta(t_spike) (ms); column 2: cross-correlation
- Panel D
- Fig3D.txt
- column 1: time (ms); columns 2-17: EPSP voltage (mV); columns 18-31: detonation voltage (mV)
- Panel E
- Fig3E.txt
- column 1: time (ms); column 2: delta(current) (pA)
- Panel F
- Fig3F.txt
- column 1: time (ms); column 2: mean current (pA); columns 3-end: individual trials
- Panels G, H
- Fig3GH.xls
Fig 4
- Panels B, D
- Fig4Bleft.txt, Fig4Bright.txt, Fig4Dleft.txt, Fig4Dright.txt
- column 1: time (ms); column 2: mean voltage (mV); columns 3-end: individual trials
- Panels E, G, J
- Fig4EGJ.xls
- Panel H
- Fig4H.txt
- column 1: time (ms); column 2: EPSP trials, pre-PhTx-74, mean voltage (mV); columns 3-41: all trials, pre-PhTx-74; column 42: all trials, post-PhTx-74, mean voltage (mV); columns 43-81: all trials, post-PhTx-74
- Panel K
- Fig4K.txt
- column 1: time (ms); column 2: pre-PhTx-74, mean current (pA); columns 3-24: all trials, pre-PhTx-74; column 25: post-PhTx-74, mean current (pA); columns 26-47: all trials, post-PhTx-74
Fig 5
- Panels B, C, E, F
- Fig5Bleft.txt, Fig5Bright.txt, Fig5Cleft.txt, Fig5Cright.txt, Fig5Eleft.txt, Fig5Eright.txt, Fig5Fleft.txt, Fig5Fright.txt
- column 1: time (ms); column 2: mean current (pA); columns 3-end: individual trials
- Panels G, H
- Fig5GH.xls
Fig 6
- Panels C, F, G
- Fig6CFG.xls
Fig 7
- Panel B
- Fig7B.txt
- column 1: time (ms); columns 2-9: voltage (mV), suprathreshold trials; columns 10-18: voltage (mV), subthreshold trials
- Panel C
- Fig7C.txt
- column 1: time (ms); column 2: total lateral inhibitory current (pA); column 3: FSI-mediated lateral inhibitory current (pA)
- Panel D
- Fig7D_total.txt
- column 1: time (ms); columns 2-end: total lateral inhibitory currents (pA)
- Fig7D_FSI.txt
- column 1: time (ms); columns 2-end: FSI-mediated lateral inhibitory currents (pA)
- Fig7D.xls
S1 Fig
- Panels A, B
- S1Fig.xls
S2 Fig
- Panels A, F, K, P
- S2FigAFKP.txt
- column 1: time (s); columns 2-5: voltage (mV) for panels A, F, K, P
- S2FigAFKPinset.txt
- column 1: time (s); columns 2-5: voltage (mV) for panels A, F, K, P
- Panels B, G, L, Q
- S2FigB.txt, S2FigG.txt, S2FigL.txt, S2FigQ.txt
- column 1: time (ms); column 2: voltage (mV); column 3: current (pA)
- S2FigBfit.txt, S2FigGfit.txt, S2FigLfit.txt, S2FigQfit.txt
- column 1: time (ms); column 2: exponential fit
- Panels C, H, M, R
- S2FigCHMR.txt
- column 1: time (ms); column 2: voltage (mV) for panels C, H, M, R
- Panels D, E, I, J, N, O, S, T
- S2FigDEIJNOST.xls
S6 Fig
- Panel A
- S6FigA.txt
- column 1: time (s): column 2: low current (pA); column 3: low voltage (mV); column 4: high current (pA); column 5: high voltage (mV)
- Panel B
- S6FigBleft.txt, S6FigBright.txt
- column 1: time (ms); column 2: mean voltage (mV); columns 3-end: individual trials
- Panel C
- S6FigC.xls
S7 Fig
- Panels A-F
- S7Fig.xls
S8 Fig
- Panels A, C, D, F
- S8FigACDF.xls
- Panel B
- S8FigB_ctrl.txt, S8FigB_NA.txt, S8FigB_NAG.txt, S8FigB_wash.txt
- column 1: time (ms); column 2: mean voltage (mV); columns 3-end: individual trials (mV)
- Panel E
- S8FigE_ctrl.txt, S8FigE_NA.txt, S8FigE_NAG.txt, S8FigE_wash.txt
- column 1: time (ms); column 2: mean current (pA); columns 3-end: individual trials (pA)
S9 Fig
- Panels B, E
- S9FigB.txt, S9FigE.txt
- column 1: time (s); column 2: low current (pA); column 3: low voltage (mV); column 4: high current (pA); column 5: high voltage (mV)
- Panels C, F
- S9FigCleft.txt, S9FigCright.txt, S9FigFleft.txt, S9FigFright.txt
- column 1: time (ms); column 2: mean voltage (mV) or current (pA); columns 3-end: individual trials
S10 Fig
- Panels A-G
- S10Fig.xls
S11 Fig
- Panels A-D
- S11Fig.xls
S13 Fig
- Panel B
- S13FigB.txt
- column 1: time (ms); columns 2-12: EPSP voltage (mV); columns 13-25: detonation voltage (mV)
- Panel C
- S13FigC.txt
- column 1: time (ms); column 2: delta(current) (pA)
- Panel E
- S13FigE.txt
- column 1: time (ms); columns 2-8: EPSP voltage (mV); columns 9-21: detonation voltage (mV)
- Panel F
- S13FigF.txt
- column 1: time (ms); column 2: delta(voltage) (mV)
S14 Fig
- Panels A-F
- S14Fig.xls
S1 Table
- S1Table.xls
S2 Table
- S2Table.xls