The extrafollicular response is sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus
Data files
Dec 08, 2022 version files 56.89 GB
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README_file_the_extrafollicular_response_is_sufficient_paper_Voss_LF_et_al_2022_final3.rtf
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Abstract
Many autoimmune diseases are characterized by germinal center (GC)-derived, affinity-matured, class-switched autoantibodies, and strategies to block GC formation and progression are currently being explored clinically. However, extrafollicular responses can also play a role. The aim of this study was to investigate the contribution of the extrafollicular pathway to autoimmune disease development. We blocked the GC pathway by knocking out the transcription factor Bcl-6 in GC B cells, leaving the extrafollicular pathway intact. We tested the impact of this intervention in two murine models of systemic lupus erythematosus (SLE): a pharmacological model based on the chronic epicutaneous application of the Toll-like receptor (TLR)-7 agonist Resiquimod (R848), and 564Igi autoreactive B cell receptor knock-in mice. The B cell-intrinsic effects were further investigated in vitro and in autoreactive mixed bone marrow chimeras. GC block failed to curb autoimmune progression in the R848 model based on anti-dsDNA and plasma cell output, superoligomeric DNA complexes, and immune complex deposition in glomeruli. The 564Igi model confirmed this based on anti-dsDNA and plasma cell output. In vitro, loss of Bcl-6 prevented GC B cell expansion and accelerated plasma cell differentiation. In a competitive scenario in vivo, B cells harboring the genetic GC block contributed disproportionately to the plasma cell output. We identified the extrafollicular pathway as a key contributor to autoimmune progression. We propose that therapeutic targeting of low-quality and poorly controlled extrafollicular responses could be a desirable strategy to curb autoreactivity, as it would leave intact the more stringently controlled and high-quality GC responses providing durable protection against infection.
Methods
Please see the README document ("README_file extrafollicular response is sufficient paper Voss LF et al 2022") and the accompanying published article: Voss LF et al. 2022. The extrafollicular response is sufficient to drive initiation of autoimmunity and early disease hallmarks of lupus. Frontiers in Immunology. Accepted. DOI: 10.3389/fimmu.2022.1021370