Re-analysis of microarray data from rapamycin resistant DLBCL cell lines
Megan Laurance (2013), Re-analysis of microarray data from rapamycin resistant DLBCL cell lines, UC San Francisco, Dataset, https://doi.org/10.7272/Q6TD9V7J
This dataset contains a re-analysis of the raw microarray data originally published by Petrich AM et al in 2012 (citation details are provided through the link to the GEO record). We were interested in re-analyzing the data because the list of differentially expressed genes that were identified when comparing rapamycin resistant DLBCL cell lines to rapamycin sensitive cell lines was not included in the original article or supplemental materials. We were interested in validating and expanding upon the findings from the original article by reevaluating the raw microarray data. Our reanalysis identified over 200 genes that were significantly differentially expressed between rapamycin resistant and sensitive cells. Importantly, our analysis highlighted a gene that was highly upregulated in rapamycin resistant cells, CD247, that was not the focus on the original publication, and is the target of a drug currently in clinical trials for refractory DLBCL. Our reanalysis also highlighted the role of SYK, a kinase upregulated in rapamycin resistant cell lines, that has a direct molecular relationship with CD247, and is a potential biomarker of drug response in DLBCL.