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Fitness consequences of biochemical adaptation in Drosophila melanogaster populations under simultaneous selection for faster pre-adult development and extended life-span

Citation

Sharma, Khushboo; Shakarad, Mallikarjun N (2021), Fitness consequences of biochemical adaptation in Drosophila melanogaster populations under simultaneous selection for faster pre-adult development and extended life-span, Dryad, Dataset, https://doi.org/10.5061/dryad.d51c5b018

Abstract

In holometabolous insect like Drosophila melanogaster, critical size is an important time point during larval life, for irreversible commitment to metamorphosis. Here, we studied the impact of restricted growth duration in terms of selection for faster pre-adult development in Drosophila melanogaster populations which resulted in the evolution of reduced critical size on adult life history traits. Selection for faster pre-adult development resulted in biochemical adaptation in larval physiology with no compromise in major biomolecules at critical size time point.  The flies from the selected populations seem to not only commit to metamorphosis on the attainment of critical size but also seem to channelize resources to reproduction as indicated by similar life-time fecundity of CS and NS flies from selected populations, while the Control CS flies significantly lower life-time fecundity compared to Control NS flies. The flies from selected populations seem to achieve longevity comparable to control flies despite being significantly smaller in size- thus resource constrained due to faster pre-adult development.

Methods

1) Life history assays- Adults- Body size measurement by dry weight measurement, Fecundity assay, longevity assay was estimated as described in research article (manuscript)

2) Biochemical estimation was performed on larval stages.

3) Statistical analysis was done on raw data.

Funding

Council for Scientific and Industrial Research, India, Award: 37(1495/11/EMR-II)

Council for Scientific and Industrial Research, India, Award: 37(1495/11/EMR-II)