#A resurrection experiment reveals reduced adaptive potential in a common agricultural weed

DOI: https://datadryad.org/dataset/doi:10.5061/dryad.2z34tmpz8

All datasheets come from a single, shared resurrection experiment conducted in a field environment. Due to vole herbivory and plant death in the field, not all plants had all traits measured, so traits measured together have their own data files (DataS1_floralMorph, DataS2_brix, DataS3_doffLC) for the sake of including all measurements. There is also a collated data file (DataS4_collated) including only plants with all traits measured for the sake of standardization across traits and multivariate analyses.

Plant ID, Population, Block, and Maternal line are shared across all data sheets (i.e., Population 1 in the DataS1 spreadsheet is the same population as Population 1 in DataS2-5).

Five .csv data files

1. DataS1_floralMorph.csv

This dataset includes floral morphology measurements from 3-5 flowers/plant in a resurrection experiment done with Ipomoea purpurea (the common morning glory). Seeds were collected from 7 populations in the SE United States at two time points, 2003 [ancestral] and 2012 [descendant], and grown together in a common garden field experiment. Floral measurements included are corolla width, corolla length, and the distance between the tallest anther and stigma (anther-stigma distance).

Columns:

• ID - ID number assigned to each individual plant measured in the resurrection experiment
• CorollaWidth - corolla width; measurement (in mm) of the diameter of the corolla
• CorollaLength - corolla length; measurement (in mm) from the base of the sepal to the outer edge of the corolla
• ASD - anther-stigma distance; difference between the length of the tallest anther (measurement in mm from the base of the sepal to the tip of the tallest anther) and the stigma (measurement in mm from the base of the sepal to the tip of the stigma)
• LeafCount - total number of leaves on a plant at the time of first flowering
• count - total number of seeds produced by a plant
• Pop - ID number assigned to each unique, naturally occurring, population from which seeds were collected
• Year - year (either 2003 or 2012) in which seeds were collected from naturally occurring populations
• ML - ID assigned to each maternal line represented from field collections
• Block - three sub-areas within the common garden representing possible spatial variation in environment across the common garden site. Population and maternal line sampling is identical within each block
• Lat - latitude for each population
• Long - longitude for each population
• relFit - relative fitness; total seed count divided by mean seed count

2. DataS2_brix.csv

This dataset includes measurements of nectar sucrose content [in degrees Brix] from 3-5 flowers/plant in a resurrection experiment done with Ipomoea purpurea (the common morning glory). Seeds were collected from 7 populations in the SE United States at two time points, 2003 [ancestral] and 2012 [descendant], and grown together in a common garden field experiment.

Columns:

• ID - ID number assigned to each individual plant measured in the resurrection experiment
• brix - measurement of the sucrose content of the nectar in a flower using the Brix scale and recorded from a pocket refractometer
• LeafCount - total number of leaves on a plant at the time of first flowering
• count - total number of seeds produced by a plant
• Pop - ID number assigned to each unique, naturally occurring, population from which seeds were collected
• Year - year (either 2003 or 2012) in which seeds were collected from naturally occurring populations
• ML - ID assigned to each maternal line represented from field collections
• Block - three sub-areas within the common garden representing possible spatial variation in environment across the common garden site. Population and maternal line sampling is identical within each block
• Lat - latitude for each population
• Long - longitude for each population
• relFit - relative fitness; total seed count divided by mean seed count

3. DataS3_doffLC.csv

This dataset includes the date of first flower (in Julian Date) and leaf count at flowering for each surviving plant [839 in total] in a resurrection experiment done with Ipomoea purpurea (the common morning glory). Seeds were collected from 7 populations in the SE United States at two time points, 2003 [ancestral] and 2012 [descendant], and grown together in a common garden field experiment.

Columns:

• ID - ID number assigned to each individual plant measured in the resurrection experiment
• JulianDate - date on which the first flower of a plant opens, synonymous with days after planting; converted into Julian Date
• LeafCount - total number of leaves on a plant at the time of first flowering
• count - total number of seeds produced by a plant
• Pop - ID number assigned to each unique, naturally occurring population from which seeds were collected
• Year - year (either 2003 or 2012) in which seeds were collected from naturally occurring populations
• ML - ID assigned to each maternal line represented from field collections
• Block - three sub-areas within the common garden representing possible spatial variation in environment across the common garden site. Population and maternal line sampling is identical within each block
• Lat - latitude for each population
• Long - longitude for each population
• relFit - relative fitness; total seed count divided by mean seed count

4. DataS4_collated.csv

This dataset includes all trait measurements (corolla width, corolla length, anther-stigma distance, nectar sucrose content, date of first flower, leaf count, and total seed count) combined together in a single dataset and restricted to plants for which there are measurements for every trait. This data comes from a resurrection experiment done with Ipomoea purpurea (the common morning glory). Seeds were collected from 7 populations in the SE United States at two time points, 2003 [ancestral] and 2012 [descendant], and grown together in a common garden field experiment.

Columns:

• ID - ID number assigned to each individual plant measured in the resurrection experiment
• Pop - ID number assigned to each unique, naturally occurring population from which seeds were collected
• Year - year (either 2003 or 2012) in which seeds were collected from naturally occurring populations
• ML - ID assigned to each maternal line represented from field collections
• Block - three sub-areas within the common garden representing possible spatial variation in environment across the common garden site. Population and maternal line sampling is identical within each block
• Lat - latitude for each population
• Long - longitude for each population
• LeafCount - total number of leaves on a plant at the time of first flowering
• count - total number of seeds produced by a plant
• CW - corolla width; measurement (in mm) of the diameter of the corolla
• CL - corolla length; measurement (in mm) from the base of the sepal to the outer edge of the corolla
• ASD - anther-stigma distance; difference between the length of the tallest anther (measurement in mm from the base of the sepal to the tip of the tallest anther) and the stigma (measurement in mm from the base of the sepal to the tip of the stigma)
• brix - measurement of the sucrose content of the nectar in a flower using the Brix scale and recorded from a pocket refractometer
• JulianDate - date on which the first flower of a plant opens, synonymous with days after planting; converted into Julian Date

5. DataS5_polTrait.csv

This dataset includes pollinator visitation data for a subset of 277 plants. Pollinator behavior surveys were conducted in 20-minute rounds with any pollinator approach or visitation to a flower recorder. For each plant observed, the average corolla width, length, anther-stigma distance, and brix were also recorded for the day of observation along with the day of first flower and leaf count for the plant.

Columns:

• Year - year (either 2003 or 2012) in which seeds were collected from naturally occurring populations
• Block - three sub-areas within the common garden representing possible spatial variation in environment across the common garden site. Population and maternal line sampling is identical within each block
• Forage - residuals from a linear model of Forage ~ Block, taken to remove block effect from the SEM; Forage represents number of times within an observing period a pollinator entered a flower
• Approach - residuals from a linear model of Approach ~ Block, taken to remove block effect from the SEM; Approach represents number of times within an observing period a pollinator approached, but did not enter, a flower
• relFit - residuals from a linear model of relFit ~ Block, taken to remove block effect from the SEM; relFit is relative fitness (total seed count divided by mean seed count)
• CWresid - residuals from a linear model of Corolla Width ~ Block taken to remove block effect from the SEM
• CLresid - residuals from a linear model of Corolla Length ~ Block taken to remove block effect from the SEM
• ASDresid - residuals from a linear model of ASD ~ Block taken to remove block effect from the SEM
• Bresid - residuals from a linear model of Brix ~ Block taken to remove block effect from the SEM
• FFresid - residuals from a linear model of Julian Date of First Flower ~ Block taken to remove block effect from the SEM
• LCresid - residuals from a linear model of Leaf Count ~ Block taken to remove block effect from the SEM

Code/Software

All analysis and generation of figures included as an R markdown file (Full_Code.Rmd). Seven external R scripts are referenced and included.