Data from: Threshold effect of growth rate on population variability of Escherichia coli cell lengths

Gangan MS, Athale CA

Date Published: October 10, 2018

DOI: https://doi.org/10.5061/dryad.2bs69.2

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Title Program to analyze E. coli cell lengths from DIC images
Description DIC cell length detection code developed in MATLAB and released into the Open Source community. This code is suited for all elongated cell types and has been optimized for the purposes of analyzing E. coli cells. All the data analyzed in the related manuscript for cell-lengths has been generated using this program.
Download //github.com/athale/ecolilenDIC (0 bytes)
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Title E. coli population cell lengths: mutants, drug-treatment and growth rates
Downloaded 1 time
Description The file includes the raw-data used to calculate the coefficient of variation of E. coli cell lengths. The data encompasses (as marked in the tabs) the growth rate modulation, mother-mother machine analysis, microc-colony analysis, multiple Hydroxyurea concentrations, Trimethoprim treatment and mutants.
Download 20170119-cellLengths-athale-gangan.xlsx (1.007 Mb)
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Title Video legend
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Description Description of the Supplementary Videos S1 through S4.
Download videoLegend-gangan.pdf (133.5 Kb)
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Title Supplementary video S1
Downloaded 4 times
Description The growth and division of a population of E. coli MG1655 cells on an agar-pad is followed in DIC (green) simultaneously with the HupA-GFP (red). The time-stamp is in units hh:mm. The arrow indicates a cell elongation event corresponding to a delay in nucleoid segregation. The scale bar is 10 μm.
Download videoS1-K12-DIC-hupAGfp.mov (1.717 Mb)
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Title Supplementary video S2
Downloaded 2 times
Description Time-lapse images of continuous culture of E. coli MG1655 cells grown in LB were acquired in DIC microscopy. The channels restricting the movement of the cells correspond to the dead-end channels of the ‘mother machine’, while the central trench corresponds to the nutrient channel. The interval between each frame is 100 seconds. The time stamp is hh:mm:ss. Scale bar: 5 μm.
Download VideoS2-LBmothermachine.mov (2.891 Mb)
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Title Supplementary video S3
Downloaded 1 time
Description Time-lapse images of E. coli MG1655 cells in continuous culture grown in the ‘mother machine’ with M9 + succinate as the medium at 37°C were recorded in DIC. Images were acquired every 120 s. The time stamp is hh:mm:ss. Scale bar: 5 μm.
Download VideoS3-M9mothermachine.mov (502.6 Kb)
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Title Supplementary video S4
Downloaded 3 times
Description DIC time series of E. coli MG1655 micro-colony originated from single mother cell and growing on agar pad at 37°C was imaged at an interval of 2 minutes between every frame. The time stamp is hh:mm:ss (Scale bar: 5 μm).
Download VideoS4-DICmicrocolony.mov (322.5 Kb)
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When using this data, please cite the original publication:

Gangan MS, Athale CA (2017) Threshold effect of growth rate on population variability of Escherichia coli cell lengths. Royal Society Open Science 4: 160417. https://doi.org/10.1098/rsos.160417

Additionally, please cite the Dryad data package:

Gangan MS, Athale CA (2017) Data from: Threshold effect of growth rate on population variability of Escherichia coli cell lengths. Dryad Digital Repository. https://doi.org/10.5061/dryad.2bs69.2
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